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bifunctional biotin maleimide probe  (Vector Laboratories)


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    Structured Review

    Vector Laboratories bifunctional biotin maleimide probe
    Bifunctional Biotin Maleimide Probe, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SP-1501-12/Biotin+(Long+Arm)+Maleimide/pm41484353-430-15-23
    Average 93 stars, based on 30 article reviews
    bifunctional biotin maleimide probe - by Bioz Stars, 2026-10
    93/100 stars

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    Labeling:

    Article Title: The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis
    Article Snippet: Caren and LacZ (namely a 3,066-base pair fragment from β-geo) were cloned into a pSP72 plasmid vector (Promega) and transcribed in vitro at 37 °C for 2 h using T7 RNA polymerase (Roche, Indianapolis, IN, USA). .. Both transcripts were biotinylated using a 5’ End-Tag Nucleic Acid Labeling system (Vector Laboratories, MB-9001, Burlingame, CA, USA) and a biotin-maleimide reagent (Vector Laboratories, SP-1501). .. Each biotinylated transcript (500 ng) was incubated with C2C12 whole-cell lysates (50 μg per sample) or total RNA extracted from C2C12 cells (1 μg per sample) using an RNAeasy mini kit (QIAGEN) in lysis buffer (10 mM Tris–HCl, 1% Triton X-100, 50 mM NaCl, 30 mM sodium pyrophosphate, 50 mM NaF, 5 mM EDTA, 0.1 mM Na 3 VO 4 , a protease inhibitor cocktail (Nacalai Tesque), and 200 U/ml RNase inhibitor, pH 7.5) at 32 °C for 1 h, incubated with Dynabeads M-270 Streptavidin (Thermo Fisher Scientific Inc) at 4 °C for 1 h with rotation, and then washed 5 times for 5 min each with 1× SSC/0.5% SDS with rotation at room temperature.

    Article Title: 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
    Article Snippet: Biotinylated RNA oligos were synthesized using a 5’EndTag kit (Vector Laboratories, MB-9001), which was performed according to the manual. .. Biotin (Long Arm) maleimide (Vector Laboratories, SP-1501-12) was used for labeling a long-armed biotin group to the 5′ end of RNA oligos (GFP-r1 or 5′tRFl Glu/CTC -mimetic), which was then purified by phenol:chloroform extraction. mRNAs for GFP and NLS-hRNASEH1-GFP were in vitro synthesized using T7 polymerase. ..

    Article Title: Nanobodies as tools for studying human frataxin biology
    Article Snippet: .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector).

    Article Title: Mechanisms of length-dependent recognition of viral double-stranded RNA by RIG-I.
    Article Snippet: .. LMW poly (I:C) was biotinylated at the 5’-end according to the protocol of 5’ EndTagTM DNA/RNA Labeling Kit (MB-9001, Vectorlabs) using thiol-reactive Biotin Maleimide label (SP-1501, Vectorlabs). ..

    Article Title: Nanobodies as tools for studying human frataxin biology.
    Article Snippet: 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius). .. For this experiment, a recombinant FXN H177C variant (> 98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius).

    other:

    Article Title: Gasdermin D pore-forming activity is redox-sensitive
    Article Snippet: Biotin-maleimide , Vector Laboratories , SP-1501-12.

    Purification:

    Article Title: 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
    Article Snippet: Biotinylated RNA oligos were synthesized using a 5’EndTag kit (Vector Laboratories, MB-9001), which was performed according to the manual. .. Biotin (Long Arm) maleimide (Vector Laboratories, SP-1501-12) was used for labeling a long-armed biotin group to the 5′ end of RNA oligos (GFP-r1 or 5′tRFl Glu/CTC -mimetic), which was then purified by phenol:chloroform extraction. mRNAs for GFP and NLS-hRNASEH1-GFP were in vitro synthesized using T7 polymerase. ..

    Extraction:

    Article Title: 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
    Article Snippet: Biotinylated RNA oligos were synthesized using a 5’EndTag kit (Vector Laboratories, MB-9001), which was performed according to the manual. .. Biotin (Long Arm) maleimide (Vector Laboratories, SP-1501-12) was used for labeling a long-armed biotin group to the 5′ end of RNA oligos (GFP-r1 or 5′tRFl Glu/CTC -mimetic), which was then purified by phenol:chloroform extraction. mRNAs for GFP and NLS-hRNASEH1-GFP were in vitro synthesized using T7 polymerase. ..

    In Vitro:

    Article Title: 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
    Article Snippet: Biotinylated RNA oligos were synthesized using a 5’EndTag kit (Vector Laboratories, MB-9001), which was performed according to the manual. .. Biotin (Long Arm) maleimide (Vector Laboratories, SP-1501-12) was used for labeling a long-armed biotin group to the 5′ end of RNA oligos (GFP-r1 or 5′tRFl Glu/CTC -mimetic), which was then purified by phenol:chloroform extraction. mRNAs for GFP and NLS-hRNASEH1-GFP were in vitro synthesized using T7 polymerase. ..

    Synthesized:

    Article Title: 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryos
    Article Snippet: Biotinylated RNA oligos were synthesized using a 5’EndTag kit (Vector Laboratories, MB-9001), which was performed according to the manual. .. Biotin (Long Arm) maleimide (Vector Laboratories, SP-1501-12) was used for labeling a long-armed biotin group to the 5′ end of RNA oligos (GFP-r1 or 5′tRFl Glu/CTC -mimetic), which was then purified by phenol:chloroform extraction. mRNAs for GFP and NLS-hRNASEH1-GFP were in vitro synthesized using T7 polymerase. ..

    Recombinant:

    Article Title: Nanobodies as tools for studying human frataxin biology
    Article Snippet: .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector).

    Article Title: Nanobodies as tools for studying human frataxin biology.
    Article Snippet: 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius). .. For this experiment, a recombinant FXN H177C variant (> 98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius).

    Variant Assay:

    Article Title: Nanobodies as tools for studying human frataxin biology
    Article Snippet: .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. For this experiment, a recombinant FXN H177C variant (>98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector).

    Article Title: Nanobodies as tools for studying human frataxin biology.
    Article Snippet: 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius). .. For this experiment, a recombinant FXN H177C variant (> 98% pure) was labeled with a bifunctional biotin-maleimide probe of long arm Cat# SP-1501-12 (Vector). .. 72 Biolayer Interferometry Experiments The experiments were carried out using a BLItz instrument (Sartorius).



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